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Volume 4 Issue 1

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Open Access online publishing journal from Africa

ISSN; 2411-1376 

An African Official Scientific journal

​Original Research Paper - 1

A Study on Pharmacological Evaluation for Hypoglycemic Property of Hibiscus rosa sinensis Leaves Extract
         Mounica V*, Gopala Krishna Ch, Ashok Kumar D, Sudhakar babu A M S
Abstract:
Background: The hibiscus rosa sinensis leaves belongs to the family Malvaceae is found mainly in India. The hibiscus rosa sinensis leaves reported to contain flavonoids, alkaloids, tannins, saponins and carbohydrates
Objective: A Study on pharmacological evaluation for hypoglycemic property of hibiscus rosa sinensis leaves
Methods: The extract of dried leaves of hibiscus rosa sinensis leaves were screened for their effects on Hypoglycmic activity in normal rats.  The extracts were tested for hypoglycemic activity in  normal  rat and also extracts are screened for their effects on serum glucose level in glucose over loaded rat .The hypoglycemic potential of extracts was evaluated in normal healthy rats.
Result: The plant leaves extracts exhibited  hibiscus rosa sinensis effect in glucose loaded rats, rabbits.  Oral admisntration of hibiscus rosa sinensis leaves for 21 days significantly reduced blood glucose level.
Conclusion: The study reveals for the first time the hypoglycemic activity of hibiscus rosa sinensis leaves in both glucose fed normal rats.  Acute and chronic studies proven that root extracts showed hibiscus rosa sinensis  effect in normal rats.

Key words: Diabetes mellitus, hypoglycemic, hibiscus rosa sinensis leaves, OGTT, herbal extracts, hypoglycaemia
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Original Research Paper – 2

Biosynthesis of silver nanoparticles by fungi isolated from municipal waste
       A. Behera, S.S. Panda*, N. C. Rout and N. K. Dhal

Abstract:
A total of 25 fungal species were isolated from soil samples using soil dilution techniques collected from municipal waste located in Bhubaneswar, Odisha, India. Out of which two fungal isolates were found to be responsible for the production of silver nanoparticles. The synthesized nanoparticles were characterized by UV-Vis Spectrophotometer and the maximum absorbance was found to be at 414 and 420nm, respectively. The particle size and shape was studied by Scanning Electron Microscope (SEM), Field Emission Scanning Electron Microscope (FESEM) and Transmission Electron Microscope (TEM). The nature of the nanoparticles was identified by Elemental analysis (EDAX). The synthesized nanoparticles are identified as spherical, rod as well as irregular shaped having size of 48-68nm. The synthesis of nanoparticles by biological method has greater advantages over the physical and chemical methods, as it is environmentally safe or eco-friendly and economically one. Biosynthesis of silver nanoparticles is the ecofriendly, safe and cost effective way and its antibacterial properties also used in clinical purposes to cure or minimizing the diseases

Keywords: Silver nanoparticles, biological synthesis, isolated fungi, Scanning Electron Microscope, Transmission Electron Microscope, Field Emission Scanning Electron Microscope

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Original Research Paper – 3

Characterization of Protein from Momordica dioica Seeds for Trypsin Inhibitory Activity and its Mass Spectrometric Analysis

​​          Jinitha Varghese*, Archana Gajbhiye

Abstract:
Serine protease inhibitors have been reported from several species of Momordica and trypsin is one of the representative enzymes of serine proteases. The present study was performed to characterize the seed protein from Momordica dioica for its antitrypsin potential and antifungal activity. BAEE (N-α- Benzoyl- L- arginine ethyl ester hydrochloride) substrate based method was used for analysis of antitrypsin activity in seed protein. 94.91% of inhibition was obtained in crude extract, whereas the purified protein of Momordica dioica with trypsin inhibitory activity (MdTi) confirmed 73.99% of inhibition. Antifungal activity was checked in both crude extract as well as in purified protein MdTi against Rhizoctonia solani, Fusarium solani, Aspergillus niger, Rhizopus oryzae and Candida albicans. No antifungal activity could be confirmed in crude extract and the purified protein MdTi. The MALDI MS analysis of MdTi was performed for identification of the protein. The MS/MS ion search in Swissprot/Mascot database performed using the data obtained in MALDI MS showed that the inhibitor is similar to the squash type trypsin inhibitor of Momordica cochinchinensis.

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Volume 3 Issue 2