Version 4.200201

A431 (human, skin, carcinoma, squamous cell)

Available in the following LABORATORY:

• CAMR Centre for Applied Microbiology & Research (ECACC, Salisbury, Wiltshire)
  EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + Bovine Insulin + 10% Fetal Bovine Serum (FBS). Split confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. Karyotype: Hypertriploid
  Hazard: CX
  Derived from an 85 year old female with epidermal carcinoma. The cells carry large numbers of EGF binding sites and is an indicator cell for anti-TGF binding.
  Further information
  Research council deposit: No
  Biomed_1 Cell characteristics: 1)beside high expression of EGF receptor, the cell expresses receptor for hepatocyte growth factor (22), and for a new human receptor tyrosine kinase HEK 2 (3). 2)expresses selectin ligands (1) Applications: 1)Study of growth factor action and binding: EGF (5, 7, 12, 17, 19, 28, 36), HGF (22), TGF alpha (5, 25, 27). 2)Study of EGF receptor: EGF interaction (5, 19, 28), down regulation (42), and distribution (35, 37), tyrosine kinase activity (2, 7, 39), protein phosphorylation (6, 17, 18). 3)Source of EGF receptor: radioreceptor assay (33). 4)Screening and study of drugs and toxins: mechanism of action (20, 21, 27), activity and resistance (24), immunotoxin activity (34). 5)Study of regulation of protein expression (6, 10, 31); cell proliferation (5, 12, 23). 6)Purification and characterization of MAP kinase kinases (4, 29). 7)Study of cell-division cycle proteins (14, 30). Bibliography: (1) AMERICAN JOURNAL OF PATHOLOGY, 143 (4) 1220-5 /1993 (2) ONCOGENE, 8 (10) 3141-7 /1993 (3) ONCOGENE, 8 (10) 2857-62 /1993 (4) PNAS USA, 90 (1) 5143-7 /1993 (5) CANCER RESEARCH, 53 (18) 4322-8 /1993 (6) JOURNAL OF CELL SCIENCE, 105 ( Pt 1) 219-31 /1993 (7) CELL, 74 (3) 565-75 /1993 (8) INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, VIROLOGY, PARASITOLOGY AND INFECTIOUS DISEASES, 278 (2-3) 296-305 /1993 (9) CELLULAR IMMUNOLOGY, 149 (2) 291-302 /1993 (10) JOURNAL OF CELL SCIENCE, 104 ( Pt 2) 289-96 /1993 (11) JOURNAL OF BIOMATERIALS SCIENCE, POLYMER EDITION, 4 (3) 235-43 /1993 (12) AMERICAN JOURNAL OF PATHOLOGY, 142 (4) 1141-53 /1993 (13) RECENT PROGRESS IN HORMONE RESEARCH, 48 511-6 /1993 (14) JOURNAL OF BIOLOGICAL CHEMISTRY, 267 (32) 22860-8 /1992 (15) JOURNAL OF HISTOCHEMISTRY AND CYTOCHEMISTRY, 41 (2) 313-7 /1993 (16) JOURNAL OF BIOLOGICAL CHEMISTRY, 267 (35) 25098-103 /1992 (17) MOLECULAR AND CELLULAR BIOLOGY, 12 (12) 5843-56 /1992 (18) MOLECULAR AND CELLULAR BIOLOGY, 12 512) 5816-23 /1992 (19) JOURNAL OF BIOLOGICAL CHEMISTRY, 267 (33) 24034-40 /1992 (20) JOURNAL OF BIOLOGICAL CHEMISTRY, 267 (32) 23427-33 /1992 (21) JOURNAL OF BIOCHEMISTRY, 112 (2) 386-90 /1992 (22) EXPERIMENTAL CELL RESEARCH, 202 (2) 423-31 /1992 (23) EXPERIMENTAL CELL RESEARCH , 202 (2) 386-90 /1992 (24) ANTI-CANCER DRUGS , 3 (3) 245-52 /1992 (25) ANALYTICAL BIOCHEMISTRY, 203 (1) 70-5 /1992 (26) BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 187 (1) 152-7 /1992 (27) ONCOLOGY RESEARCH, 4 (4-5) 193-200 /1992 (28) PNAS USA, 89 (16) 7801-5 /1992 (29) JOURNAL OF BIOLOGICAL CHEMISTRY, 267 (20) 14373-81 /1992 (30) JOURNAL OF BIOLOGICAL CHEMISTRY, 267 (20) 13943-51 /1992 (31) JOURNAL OF DERMATOLOGY ,19 (2) 78-81 /1992 (32) CANCER RESEARCH, 52 (13) 3515-20 /1992 (33) ACTA PAEDIATRICA ,81 (4) 287-91 /1992 (34) CANCER RESEARCH , 52 (11) 3189-93 /1992 (35) INVESTIGATIVE OPHTALMOLOGY AND VISUAL SCIENCE, 33 (6) 2053-62 /1992 (36) BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 183 (3) 1216-23 / 1992 (37) BIOCHEMISTRY, 31 (9) 2459-63 /1992 (39) BIOCHEMICAL JOURNAL, 281 ( Pt 1) 107-14 /1992 (40) JOURNAL OF VIROLOGY, 66 (2) 824-30 /1992 (41) JOURNAL OF CELLULAR PHYSIOLOGY, 150 (1) 180-7 /1992 (42) JOURNAL OF VIROLOGY, 66 (1) 197-203 /1992
  Price_code: A
  

Availability in cell line catalogues: ATCC CRL 1555; DSMZ ACC 91;

Bibliographic references:

• J Nat Cancer Inst 1973;51:1417

By Beatrice...